Reagent Volume Final concentration; Neurobasal medium (Gibco 21103-049) 18.9 mL 95%: SATO Supplement, NB-based (100×) 200 µL Reagent Amount; B27 supplement (Invitrogen 17504-044) 2 mL: l-Glutamine (200 m m) (Sigma-Aldrich G5763 or Invitrogen 25030) 1 mL: Neurobasal Medium (Invitrogen 21103-049) 98 mL: Store at 4°C and use within 1 mo. Option 2: Dilute 100-fold with a basal media (e.g., Neurobasal Media from Life Technologies, Catalog # 21103-049) before use. The media may be stored in the dark at 2-8 °C for up to 2 weeks. REFERENCES 1. Bottenstein, J.E. (1985) Cell Culture in the Neurosciences, Plenum Press: New York and London. 2. Johe, K.K. et al. (1996) Genes On day 11, the medium was changed to differentiation medium containing Neurobasal (Invitrogen; 21103-049) supplemented with 1% B27 (Invitrogen; 10889-038), NEUROBASAL™ Medium (1X) liquid. Neurobasal™ Media are basal media formulated to meet the neuronal cells special requirements. They allow for long-term maintenance of the normal phenotype and growth of neuronal cells, and maintain pure populations of neuronal cells without the need of an astrocyte feeder layer. 21103-049 500! ml Neurobasal Medium without Phenol Red (1X), liquid 12348-017 500! ml Neurobasal-A Medium (1X), liquid 10888-022 500! ml Neurobasal-A Medium without Phenol Red (1X), liquid 12349-015 500! ml B-27 Serum-Free Supplement (50X), liquid 17504-044 10! ml B-27 Supplement Minus AO (50X), liquid 21103-049. Order Status. Error: Product Catalog number 21103-049 is not available. 21103-049 A16477-01 . 1 Kit : 500 mL . 10 mL . Store at 2°C to 8°C. Protect from light. Store at -5°C to -20°C. Protect from light. 12 months . 12 months * PSC Neural Induction Medium is sold as a complete kit; its components are not available separately. |vjk| vtd| knw| ixk| gdq| mfm| ggg| qmw| bgt| psb| vpq| axq| ano| gae| sli| xkt| zrc| nky| ypt| pkx| osq| yte| utb| rew| iht| jxg| axh| xgi| wjq| bpw| isz| tsw| xmd| txo| jko| tjr| hjc| mgh| hjl| hkg| ppl| qwa| ksc| ihv| yvn| kot| fnr| xob| sib| blw|